![]() ![]() These results suggest CDT-1 is a more effective cellobiose transporter than CDT-2 for engineering S. cerevisiae appears to be optimum for cellobiose fermentation. While CDT-2 is expected to have energetic benefits, the expression levels and kinetic properties of CDT-1 in S. A strain expressing CDT-1 and GH1-1 (DCDT-1G) showed faster cellobiose fermentation than the strain expressing CDT-2 and GH1-1 (DCDT-2G) under various culture conditions with different medium compositions and aeration levels. Cellobiose transport assays with the resulting strains indicated that CDT-1 is a proton symporter while CDT-2 is a simple facilitator. ![]() cerevisiae along with an intracellular β-glucosidase (GH1-1). In this study, CDT-1 and CDT-2, which are hypothesized to transport cellodextrin with distinct mechanisms, were introduced into S. crassa, but their kinetic properties and efficiency for cellobiose fermentation have not been studied in detail. Two cellodextrin transporters (CDT-1 and CDT-2) were previously identified in N. cerevisiae, a fungal cellodextrin-utilizing pathway from Neurospora crassa consisting of a cellodextrin transporter and a cellodextrin hydrolase has been introduced into S. To facilitate cellodextrin utilization by S. Direct fermentation of cellodextrins instead of glucose is advantageous because glucose inhibits cellulase activity and represses the fermentation of non-glucose sugars present in cellulosic hydrolyzates. Saccharomyces cerevisiae can be engineered to ferment cellodextrins produced by cellulases as a product of cellulose hydrolysis. ![]()
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